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Ac-Angiotensinogen (1-14), porcine is an N-terminally acetylated 14-residue peptide derived from the porcine angiotensinogen sequence. It is used as a defined synthetic substrate in renin research and studies of the first enzymatic step of the renin–angiotensin system.
The peptide contains the angiotensin I sequence within its N-terminal region together with downstream residues surrounding the renin cleavage site.
Product Information
| Property | Specification |
|---|---|
| Product Name | Ac-Angiotensinogen (1-14), Porcine |
| CAS No. | 66641-26-7 |
| Sequence | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser |
| One-Letter Sequence | Ac-DRVYIHPFHLLVYS |
| Peptide Length | 14 amino-acid residues |
| Molecular Formula | C87H125N21O21 |
| Molecular Weight | Approximately 1801.05 Da |
| N-Terminus | Acetylated |
| Primary Research Enzyme | Renin |
| Research Areas | Renin kinetics, RAS biology, inhibitor screening |
| Purity | Crude to 98% |
Renin Substrate Architecture
Renin initiates the classical renin–angiotensin cascade by cleaving angiotensinogen and releasing angiotensin I.
The porcine 1–14 sequence contains residues surrounding this natural cleavage region, allowing enzyme recognition to be studied using a chemically defined peptide instead of the full-length angiotensinogen protein.
N-terminal acetylation changes the terminal chemistry of the synthetic substrate and should be documented when kinetic data are compared with non-acetylated angiotensinogen fragments.
Species-Specific Sequence Context
The porcine sequence is:
Ac-DRVYIHPFHLLVYS
The first ten residues correspond to the porcine Angiotensin I region. Residues downstream of this segment provide additional sequence context around the renin cleavage site.
We recommend matching substrate species when a study is designed to compare renin specificity across human, porcine or rodent enzymes because sequence differences can influence kinetic behavior.
Research Applications
Ac-Angiotensinogen (1-14), porcine can be used in renin activity measurements, enzyme kinetic studies, substrate-recognition experiments and screening of renin-directed inhibitors.
For quantitative assays, substrate concentration should be selected with reference to the enzyme construct and assay conditions rather than transferred directly from a different species or substrate format.
Quality Considerations
Sequence identity, N-terminal acetylation, molecular mass and chromatographic purity are relevant specifications for reproducible renin assays.
Researchers can review our Recommended Peptide Purity guidance when planning an enzyme assay.
Frequently Asked Questions
What enzyme cleaves angiotensinogen-derived substrates?
Renin is the principal enzyme that initiates the classical RAS pathway by cleaving angiotensinogen to generate angiotensin I.
Is this peptide full-length angiotensinogen?
No. It is a synthetic 14-residue N-terminal fragment derived from porcine angiotensinogen.
Can human angiotensinogen substrate sequences be synthesized?
Human, porcine and other species-specific renin substrates can be evaluated through Chemical Peptide Synthesis.