Learn how to select the appropriate peptide purity for different research applications. Compare common purity levels and understand when higher peptide purity may be recommended for biochemical assays, cell-based studies, animal research, and other experimental applications.
Choosing the appropriate peptide purity depends on the sensitivity of the experiment and the intended downstream application. The table below provides a practical guide for selecting a suitable purity level for custom synthetic peptides.
| Peptide Purity | Recommended Applications |
|---|---|
| Crude / Desalted | Initial screening, sequence optimization, peptide library screening, non-sensitive qualitative studies |
| ≥75% | Antibody production, ELISA, peptide antigens, affinity purification |
| ≥85% | Epitope mapping, biochemical assays, enzyme assays, protein interaction studies |
| ≥95% | Cell-based assays, binding studies, functional studies, quantitative biochemical experiments |
| ≥98% | In vivo and animal studies, sensitive quantitative assays, and applications requiring very high peptide purity |
Intermediate purity levels including ≥80% and ≥90% are also available when a balance between experimental requirements and project budget is preferred.
Higher purity is not always necessary for every experiment. The appropriate specification should be selected according to assay sensitivity, downstream application, peptide complexity, and budget.
For projects where impurities could affect biological activity or data interpretation, a higher purity level is generally recommended.
Alan Scientific offers desalted peptides and multiple purified grades for custom research projects. A ≥99% chromatographic purity specification can be evaluated where required, subject to peptide sequence and purification feasibility. The agreed purity specification and analytical requirements are confirmed during quotation.