Substance P reversed sequence is the 11-residue peptide Met-Leu-Gly-Phe-Phe-Gln-Gln-Pro-Lys-Pro-Arg-NH₂. It contains the same amino acid composition as native Substance P but presents the residues in reverse sequence order.

The reversed peptide is useful as a sequence-specific experimental control because molecular composition and approximate size are retained while the native Substance P pharmacophore is disrupted. It can help distinguish genuine sequence-dependent effects from nonspecific effects related to peptide charge, hydrophobicity or composition.

Product Information

Same Composition, Different Sequence Order

Reversing the residue order preserves elemental composition but changes peptide directionality, side-chain topology and the location of basic and hydrophobic residues.

The native Substance P receptor-recognition motif is therefore no longer presented in its physiological sequence context.

How to Use a Reverse-Sequence Control

We recommend using reversed Substance P together with native Substance P and a vehicle control. A reverse peptide is useful for testing sequence specificity but should not automatically be assumed to be biologically inert in every assay.

Research Applications

Substance P reversed sequence can support peptide specificity controls, binding assay controls, sequence-dependence experiments and evaluation of nonspecific peptide effects.

Frequently Asked Questions

Does reversed Substance P have the same molecular formula?

Yes, when the same residues and equivalent terminal chemistry are used.

Is reversed Substance P expected to reproduce native NK1 activity?

No. The native sequence order and pharmacophore are disrupted.

Why is no CAS listed?

A reliable compound-specific registry number for this exact reversed peptide was not confirmed.

Research Use Only.