$431.00 - $2154.00
Fmoc-D-Ile-OH is an Fmoc-protected D-isoleucine building block used to introduce D-Ile during Fmoc solid-phase peptide synthesis. Its hydrophobic branched side chain does not require additional side-chain protection, allowing use in standard Fmoc deprotection and coupling workflows.
Isoleucine contains two stereogenic centers, so exact stereochemical identity matters more than for amino acids with only one chiral center. Fmoc-D-Ile-OH should not be confused with D-allo-isoleucine derivatives. Procurement teams should verify the CAS number and chiral specification when the peptide sequence requires a defined D-Ile stereoisomer.
Product Information
| Product Name | Fmoc-D-Ile-OH |
| Catalog No. | AS3030 |
| CAS No. | 143688-83-9 |
| Molecular Formula | C21H23NO4 |
| Molecular Weight | 353.41 g/mol |
| Protecting Groups | Fmoc alpha-amino protection; no side-chain protecting group |
| Primary Application | D-isoleucine incorporation in Fmoc-SPPS |
Application Scope and Assay Relevance
Primary use: Synthetic building block for site-specific D-isoleucine incorporation in Fmoc-SPPS; not intended as the protected compound for direct cell or animal dosing.
| Typical in vitro relevance | Typical downstream studies include hydrophobic-residue stereochemical scans, target-binding or enzyme-inhibition assays, serum/protease stability, and CD/NMR or other conformational studies where D-Ile versus L-Ile or allo-Ile geometry is being compared. |
| In vivo relevance | The final peptide may be advanced to pharmacokinetic, efficacy or tolerability studies when its activity has first been established. The effect of D-Ile is strongly sequence- and position-dependent. |
D-Ile is not D-allo-Ile
D-isoleucine and D-allo-isoleucine differ at one stereogenic center. They can have the same elemental composition but produce different peptide stereochemistry and conformational effects.
Purchasing and QC Considerations
Confirm CAS 143688-83-9, D-Ile stereochemistry, chemical purity, chiral purity and lot documentation. Reference peptide-synthesis grades report clear DMF solubility and separate enantiomeric-purity specifications.
Frequently Asked Questions
What is Fmoc-D-Ile-OH used for?
It is used for incorporation of D-isoleucine in Fmoc-SPPS.
Does the isoleucine side chain need protection?
No. Its hydrocarbon side chain is normally left unprotected.
Is D-Ile the same as D-allo-Ile?
No. They are distinct stereoisomers and should not be substituted without checking the required configuration.
Product Documents
A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.
Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.
Related Technical Resources
See Fmoc-D-Leu-OH for a closely related building block or synthesis resource.
Browse the complete D-Form Amino Acids category or use Alan Scientific's Chemical Peptide Synthesis service for custom peptides containing D-amino acids.