$543.00 - $543.00
Biotin-(Cys1,Lys(biotinyl)18)-Calcitonin, human is a dual-biotinylated derivative of the 32-residue human calcitonin sequence. The construct contains an N-terminal biotin label together with side-chain biotinylation at Lys18.
This two-site labeling architecture provides a higher-density affinity handle for streptavidin-based capture, detection and immobilization studies while retaining the complete calcitonin backbone.
Product Information
| Property | Specification |
|---|---|
| Product Name | Biotin-(Cys1,Lys(biotinyl)18)-Calcitonin, Human |
| Catalog No. | AS2620 |
| Sequence | Biotin-Cys-Gly-Asn-Leu-Ser-Thr-Cys-Met-Leu-Gly-Thr-Tyr-Thr-Gln-Asp-Phe-Asn-Lys(Biotin)-Phe-His-Thr-Phe-Pro-Gln-Thr-Ala-Ile-Gly-Val-Gly-Ala-Pro-NH₂ |
| One-Letter Sequence | Biotin-CGNLSTCMLGTYTQDFNK(Biotin)FHTFPQTAIGVGAP-NH₂ |
| Peptide Length | 32 amino-acid residues |
| Reported Molecular Weight | Approximately 3870.5 Da |
| Biotin Sites | N-terminus and Lys18 side chain |
| Disulfide Bond | Cys1-Cys7 |
| C-Terminus | Pro-NH₂ |
Two Independent Biotin Handles
The construct differs from a conventional singly labeled peptide because it contains two biotin groups positioned in chemically distinct environments.
This can increase opportunities for streptavidin association but may also change surface orientation, avidity and accessibility compared with mono-biotinylated calcitonin.
Affinity Capture and Immobilization
Potential applications include streptavidin pull-down experiments, immobilized ligand systems and peptide–receptor interaction studies.
For immobilization experiments, the dual label may produce multivalent interactions with streptavidin surfaces. This can be advantageous for retention but should be considered when binding kinetics or dissociation rates are interpreted.
Receptor Activity Should Be Verified Experimentally
Both the N-terminus and Lys18 are chemically modified relative to native human calcitonin. We therefore recommend treating this material as a labeled analytical probe rather than assuming that it has receptor potency identical to unmodified human calcitonin.
Correct disulfide formation, biotin incorporation and molecular mass should all be confirmed. Our Peptide Quality Control capabilities can support analytical characterization.
Frequently Asked Questions
How many biotin groups are present?
The defined construct contains two: one at the N-terminus and one on the Lys18 side chain.
Does the peptide retain the calcitonin disulfide ring?
The intended oxidized molecular form contains the native human calcitonin Cys1-Cys7 linkage.
Can a mono-biotinylated version be synthesized instead?
Alternative labeling positions and linker configurations can be evaluated through Chemical Peptide Synthesis.