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Biotin-Angiotensin I, human is an N-terminally biotinylated derivative of the human Angiotensin I decapeptide. It combines the native DRVYIHPFHL sequence with a biotin affinity handle for capture, detection and peptide-processing studies.
The C-terminal His-Leu region recognized during ACE-dependent conversion remains intact, while the N-terminal chemistry is modified by biotinylation.
Product Information
| Property | Specification |
|---|---|
| Product Name | Biotin-Angiotensin I, Human |
| Catalog No. | AS2622 |
| Sequence | Biotin-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu |
| One-Letter Sequence | Biotin-DRVYIHPFHL |
| Peptide Length | 10 amino-acid residues |
| Molecular Formula | C72H103N19O16S |
| Molecular Weight | Approximately 1522.81 Da |
| Modification | N-terminal biotin |
| Research Areas | ACE processing, affinity capture, RAS peptide metabolism |
Biotin Placement and ACE Processing
Native human Angiotensin I is converted to Angiotensin II through removal of its C-terminal His-Leu dipeptide by angiotensin-converting enzyme.
Because biotin is positioned at the opposite end of the sequence, this construct retains the native C-terminal ACE cleavage region. Enzymatic processing would generate a biotin-containing Angiotensin II-derived product while releasing His-Leu.
We recommend experimentally validating enzyme kinetics rather than assuming that N-terminal biotinylation leaves the catalytic parameters of native Angiotensin I completely unchanged.
Affinity-Based Research Applications
The biotin group allows interaction with streptavidin-based reagents and surfaces, supporting peptide capture, immobilization and detection workflows.
This makes Biotin-Angiotensin I useful for experiments that combine RAS peptide processing with affinity-based analytical methods.
Experimental Considerations
Biotinylated and unlabeled Angiotensin I should be treated as distinct molecular forms when quantitative ACE kinetics are compared. Reporter accessibility can also depend on the assay architecture and streptavidin surface density.
Custom labeling positions, spacers and related RAS peptides can be evaluated through our Chemical Peptide Synthesis capabilities.
Frequently Asked Questions
Where is biotin attached in this peptide?
The biotin group is attached at the N-terminus of the human Angiotensin I sequence.
Does Biotin-Angiotensin I contain the native ACE cleavage region?
Yes. The C-terminal His-Leu region of the Angiotensin I sequence is retained.