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[Tyr(P)4] Angiotensin II is a phosphotyrosine-containing analog of Angiotensin II in which the Tyr4 residue carries an O-phosphate group.
The modification introduces a defined phosphotyrosine site into the eight-residue Angiotensin II scaffold, making the peptide useful for phosphatase research, phosphotyrosine substrate studies and investigation of how Tyr4 phosphorylation changes Angiotensin II chemistry and function.
Product Information
| Property | Specification |
|---|---|
| Product Name | [Tyr(P)4] Angiotensin II |
| Synonym | Angiotensin II Substrate |
| CAS No. | 129785-85-9 |
| Sequence | Asp-Arg-Val-Tyr(PO3H2)-Ile-His-Pro-Phe |
| Sequence Shortening | DRV-pY-IHPF |
| Peptide Length | 8 amino-acid residues |
| Molecular Formula | C50H72N13O15P |
| Molecular Weight | Approximately 1126.20 Da |
| Modification | O-Phosphorylation at Tyr4 |
| Research Areas | Phosphotyrosine biology, phosphatase assays, angiotensin analog research |
Tyr4 Phosphorylation
Native human Angiotensin II contains Tyr4 but no covalently attached phosphate group. [Tyr(P)4] Angiotensin II adds a phosphate to the phenolic hydroxyl of this residue.
The modification increases molecular mass and introduces additional negative charge into a region that contributes to Angiotensin II molecular recognition.
We recommend identifying this material explicitly as [Tyr(P)4] Angiotensin II in experimental records because its molecular form is distinct from unmodified Angiotensin II.
Use as a Phosphotyrosine Substrate
Phosphorylated Angiotensin II has been used historically as a defined phosphotyrosine-containing substrate for studying phosphatase activity.
The peptide is particularly convenient for this type of work because Angiotensin II contains tyrosine but does not contain serine or threonine, allowing phosphorylation state to be assigned to the Tyr residue in the defined synthetic analog.
Vascular Research Context
Early synthetic studies also evaluated the biological activity of [Tyr(P)4] Angiotensin II and reported vascular responses in experimental models. Phosphorylation therefore should not be assumed to convert the peptide into an analytically useful but biologically inert molecule.
Native and phosphorylated Angiotensin II should be compared as separate molecular entities when receptor-dependent effects are measured.
Quality Considerations
For a phosphorylated peptide, molecular identity requires confirmation of both the peptide backbone and phosphorylation state. HPLC purity alone does not establish correct phosphotyrosine incorporation.
Our Peptide Quality Control capabilities support mass spectrometry and chromatographic characterization according to the selected specification.
Frequently Asked Questions
Where is Angiotensin II phosphorylated in this product?
The phosphate group is attached to Tyr4.
Is [Tyr(P)4] Angiotensin II chemically identical to Angiotensin II?
No. Tyr4 phosphorylation adds a phosphate group, changing molecular mass, charge and chemical properties.
Can other phosphorylated peptides be synthesized?
Site-specific phosphoserine, phosphothreonine and phosphotyrosine peptides can be evaluated through Chemical Peptide Synthesis.