Ac-Gly-Pro-OH; CAS 99783-45-6 is N-acetylglycyl-L-proline, an N-capped Gly-Pro dipeptide containing the conformationally distinctive proline residue.

Proline changes peptide-backbone geometry and can populate cis/trans amide states differently from conventional residues. A capped Gly-Pro model can therefore be useful in physical-organic, conformational and analytical studies without a free N-terminal charge.

Product Information

Proline Conformation as the Main Experimental Variable

The Gly-Pro bond introduces conformational behavior that differs from most Xaa-Xaa peptide bonds. N-acetyl capping helps reduce terminal charge effects, making the compound useful for cis/trans, solvation and chromatographic model studies.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Fit and Sample Preparation

For comparative or quantitative work, review the lot-specific purity method together with identity data. Chromatographic purity is method-dependent and should be interpreted separately from molecular-mass confirmation or gravimetric vial content.

Solvent and working concentration should be validated for the intended assay. Solution history can influence small peptide models through aggregation, oxidation or concentration-dependent behavior even when the dry material meets its release specification.

Product Documents

MSDS - Ac-Gly-Pro-OH (AS4037)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is Ac-Gly-Pro-OH?

It is N-acetylglycyl-L-proline, a defined research compound with sequence or structural identity Ac-Gly-Pro.

What is the main value of this product?

Its main value is as a chemically defined reference for proline-containing peptide model.

What should be checked before quantitative use?

Confirm lot-specific identity, purity basis and analytical documentation appropriate to the method.

Research Use Only.