H-Ala-Sar-OH; CAS 69875-15-6 is L-alanyl-sarcosine, a dipeptide containing sarcosine (N-methylglycine) at the C-terminal position. The N-methyl substitution changes local amide geometry and hydrogen-bonding capability relative to Ala-Gly.

That structural difference makes Ala-Sar useful in transport, peptidase and analytical model studies where an N-methyl amino-acid residue is part of the experimental design.

Product Information

N-Methyl Residue Effects

Sarcosine removes one backbone N-H donor and changes steric environment at the peptide bond. This makes Ala-Sar a useful small model for N-methylation effects in peptidase, transport or physicochemical studies.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Use and Lot Documentation

For qualitative identification or method development, sequence identity, expected molecular mass and chromatographic purity may be sufficient. Quantitative use can require additional lot-specific information such as an assigned purity or content basis and a preparation protocol appropriate to the analytical method.

HPLC purity and mass-spectrometric identity answer different questions and should not be treated as interchangeable. Solubility and solution stability should also be validated at the concentration and solvent conditions used in the experiment when no product-specific solution data are available.

Product Documents

MSDS - H-Ala-Sar-OH (AS4142)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is H-Ala-Sar-OH?

It is L-alanyl-sarcosine; Ala-Sar, a defined research compound with sequence or structural identity Ala-Sar.

What is the main value of this product?

Its main value is as a chemically defined reference for n-methyl amino-acid dipeptide reference.

What should be checked before quantitative use?

Confirm lot-specific identity, purity basis and analytical documentation appropriate to the method.

Research Use Only.