H-Leu-Leu-OH (L-leucyl-L-leucine; CAS 3303-31-9) is a hydrophobic L,L-dipeptide and a naturally occurring metabolite/reference compound. The adjacent leucine side chains create a compact model of a dileucine sequence environment.

Leu-Leu has been used in ligand-binding and motif-function studies. It is also useful as an analytical or biochemical reference where exact sequence and L,L stereochemistry matter.

Product Information

Dileucine Motif and Binding Studies

Leu-Leu has been used to investigate dileucine motif functionality and is sold for ligand-binding applications by major suppliers. A free dipeptide is still a simplified model, so results should not be equated automatically with a dileucine motif embedded in a membrane or full-length protein.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Use and Lot Documentation

For qualitative identification or method development, sequence identity, expected molecular mass and chromatographic purity may be sufficient. Quantitative use can require additional lot-specific information such as an assigned purity or content basis and a preparation protocol appropriate to the analytical method.

HPLC purity and mass-spectrometric identity answer different questions and should not be treated as interchangeable. Solubility and solution stability should also be validated at the concentration and solvent conditions used in the experiment when no product-specific solution data are available.

Product Documents

MSDS - H-Leu-Leu-OH (AS4160)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is H-Leu-Leu-OH?

It is L-leucyl-L-leucine; Leu-Leu, a defined research compound with sequence or structural identity Leu-Leu.

What is the main value of this product?

Its main value is as a chemically defined reference for dileucine motif model.

What should be checked before quantitative use?

Confirm lot-specific identity, purity basis and analytical documentation appropriate to the method.

Research Use Only.