H-Leu-βNA·HCl is L-leucine β-naphthylamide hydrochloride, a reporter-linked amino-acid substrate used in enzyme-assay and histochemical research.

Compared with H-Leu-AMC·HCl, β-naphthylamide provides a different reporter system with different optical detection and derivatization options.

Product Information

Application Scope in Enzyme Assays

Leu-βNA derivatives are used to study aminopeptidase/protease activity when enzymatic hydrolysis releases β-naphthylamine. The reporter can be detected directly or through coupled color-forming chemistry depending on the assay design.

Assay Development Considerations

Signal intensity depends on enzyme specificity, pH, reporter detection method, substrate concentration and background hydrolysis. We recommend validating linear range and nonspecific cleavage rather than transferring conditions from an AMC assay unchanged.

Procurement and QC

Confirm CAS 893-36-7, C16H21ClN2O, MW 292.81 g/mol and hydrochloride salt state. Free β-naphthylamine impurity can affect assay background, so HPLC and reporter-baseline checks are useful for quantitative work.

Specify the enzyme system, detection format and purity requirement when requesting custom quantities.

Product Documents

A Safety Data Sheet (SDS / MSDS) is available for this product to support laboratory handling, storage and safety assessment.

H-Leu-βNA·HCl MSDS

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is βNA?

βNA denotes β-naphthylamide/β-naphthylamine reporter chemistry.

Is the detection chemistry identical to AMC?

No. βNA and AMC use different reporter properties and assay readouts.

What is a common application?

Aminopeptidase or protease substrate assays and related histochemical detection.

Related Technical Resources

Research Use Only.