$1206.00 - $3618.00
H-Leu-AMC·HCl couples L-leucine to 7-amino-4-methylcoumarin (AMC) and is supplied as the hydrochloride salt. Cleavage of an appropriate Leu–AMC amide can release the fluorescent AMC reporter, making this chemistry useful in enzyme-assay design.
For a different aromatic reporter format, Alan Scientific also lists H-Leu-βNA·HCl, which uses β-naphthylamide rather than AMC.
Product Information
| Product Name | H-Leu-AMC·HCl |
| Catalog No. | AS4175 |
| CAS No. | 62480-44-8 |
| Molecular Formula | C16H21ClN2O3 |
| Molecular Weight | 324.81 g/mol |
| Material / Building Block Type | Leucine–7-amino-4-methylcoumarin hydrochloride |
| Primary Applications | Fluorogenic protease and aminopeptidase assay development; enzyme-activity screening; substrate-specificity studies; AMC reporter peptide synthesis |
Application Scope in Fluorogenic Protease Assays
Leu-AMC chemistry is used to develop substrates for aminopeptidases and proteases that recognize a Leu-containing cleavage site. It can support kinetic assay optimization, enzyme-activity screening, inhibitor testing and substrate-specificity studies when the enzyme system is appropriate.
Reporter Chemistry and Signal Generation
AMC is weakly fluorescent while amide-bound relative to free reporter in many assay formats; enzymatic release increases the measurable signal. Excitation/emission settings, buffer pH, substrate concentration and enzyme specificity should be validated for the actual assay.
Procurement and Analytical Control
Confirm CAS 62480-44-8, C16H21ClN2O3, MW 324.81 g/mol and HCl salt state. For assay work, purity, free AMC background and lot-to-lot fluorescence baseline can be more important than nominal mass alone.
For longer fluorogenic substrates containing an AMC reporter, Alan Scientific can provide custom peptide synthesis.
Product Documents
A Safety Data Sheet (SDS / MSDS) is available for this product to support laboratory handling, storage and safety assessment.
Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.
Frequently Asked Questions
What does AMC stand for?
7-amino-4-methylcoumarin.
Is H-Leu-AMC suitable for every protease?
No. The enzyme must recognize and cleave the relevant Leu–AMC substrate context.
Why is reporter background important?
Residual free AMC can elevate baseline fluorescence and distort kinetic measurements.