H-Gly-Gly-Gly-OH (triglycine; CAS 556-33-2) is the linear tripeptide Gly-Gly-Gly. Its small, achiral sequence makes it a widely used reference peptide and a practical substrate/model for aminopeptidase and peptide-method studies.

Because triglycine is commercially common, visibility depends less on repeating the exact name and more on giving scientists useful context around enzyme-substrate work, analytical method development and the distinction between triglycine and longer glycine oligomers.

Product Information

Biochemical and Analytical Use

Triglycine is used as a compact peptide standard and has been described as a substrate for tripeptide aminopeptidase. It is also useful in method development because the sequence contains no side-chain chromophores or stereocenters, making peptide-bond chemistry the dominant structural feature.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Use and Lot Documentation

For qualitative identification or method development, sequence identity, expected molecular mass and chromatographic purity may be sufficient. Quantitative use can require additional lot-specific information such as an assigned purity or content basis and a preparation protocol appropriate to the analytical method.

HPLC purity and mass-spectrometric identity answer different questions and should not be treated as interchangeable. Solubility and solution stability should also be validated at the concentration and solvent conditions used in the experiment when no product-specific solution data are available.

Product Documents

MSDS - H-Gly-Gly-Gly-OH (AS4080)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is H-Gly-Gly-Gly-OH?

It is Triglycine; glycylglycylglycine, a defined research compound with sequence or structural identity Gly-Gly-Gly.

Is triglycine used as an enzyme substrate?

Yes. It has been described as a substrate for tripeptide aminopeptidase and is also widely used as a simple peptide reference.

Is triglycine the same as pentaglycine?

No. Triglycine contains three glycine residues, while pentaglycine contains five.

Research Use Only.