gp100 (178–186) is a 9-amino-acid PMEL-derived peptide with the sequence MLGTHTMEV. It has been characterized as an HLA-A2-associated gp100 epitope and studied in peptide-pulsed dendritic-cell systems for the generation and analysis of melanoma-reactive cytotoxic T-cell responses.

The peptide is particularly interesting because an overlapping 10-mer, gp100 (177–186), adds a single N-terminal alanine while preserving the remainder of the sequence.

Product Information

A 9-mer Within an Overlapping gp100 Epitope Region

Both MLGTHTMEV (178–186) and AMLGTHTMEV (177–186) have been described in HLA-A2-associated gp100 research. Their one-residue difference provides a useful system for examining how peptide length and N-terminal extension influence antigen presentation and T-cell recognition.

Researchers interested in this comparison can pair AS2688 with gp100 (177–186), AS2687.

Experimental Use

The 9-mer can be used in HLA-A2 peptide-loading experiments, CTL stimulation studies, antigen-presentation assays, pMHC-related research, and comparative analysis of overlapping gp100 epitopes.

Because peptide response is dependent on HLA background and assay system, concentration and purity should be determined according to the experimental model rather than treated as universal conditions.

Analytical Quality

For experiments comparing closely related epitopes, consistent identity and purity across peptides can be particularly important. Analytical HPLC and mass spectrometry provide complementary tools for confirming material used across matched experiments.

See Peptide Quality Control for additional analytical information.

Storage and Handling

Store the lyophilized peptide according to the batch-specific specification. For comparative assays, use consistent reconstitution, aliquoting, and storage procedures across peptide groups where practical.

Research Use Only

gp100 (178–186) is supplied for research use only. It is not intended for therapeutic, or human use.