γ-L-Glutamyl-4-nitroanilide is a chromogenic γ-glutamyl substrate commonly abbreviated Glu-p-NA. The molecule couples an L-glutamyl group to p-nitroaniline, allowing enzyme-mediated cleavage or transfer reactions to be followed through release or formation of a chromophoric product.

This compound is primarily used in enzyme-assay chemistry rather than as an amino-acid building block. Its use depends on the enzyme system, substrate concentration, buffer composition, acceptor substrate and detection method.

Product Information

Chromogenic Substrate Role

γ-Glutamyl-p-nitroanilide is widely associated with γ-glutamyltransferase (GGT) assay chemistry and has also appeared in glutamyl-enzyme studies. The p-nitroanilide group provides an analytically convenient reporter compared with an unlabeled glutamyl substrate.

Researchers looking for assay-oriented reagents can also review the enzyme substrate collection rather than relying only on the amino-acid category.

Assay Selection Considerations

Enzyme activity measured with a chromogenic substrate depends on substrate concentration, buffer composition, pH, temperature, acceptor components, and optical readout. Literature assay conditions should therefore be matched to the enzyme preparation rather than treated as universal.

For procurement, the substrate name, CAS, purity, hydrate state, and lot-specific COA should be checked because supplier listings can describe anhydrous and hydrated material differently.

Identity and Hydrate-State Caution

The molecular formula C11H13N3O5 and molecular weight 267.24 g/mol correspond to the anhydrous form. Because hydrated forms may appear under closely related names, confirm the exact molecular form against the lot-specific COA and SDS when molecular-weight accuracy is important.

Product Documents

MSDS - γ-L-Glutamyl-4-Nitroanilide (AS2109)

The Safety Data Sheet provides product identification, hazard information, handling and storage guidance, exposure controls, transport information, and regulatory information for laboratory use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is Glu-p-NA?

Glu-p-NA is a common abbreviation for γ-L-glutamyl-p-nitroanilide, a chromogenic γ-glutamyl substrate.

What enzymes use γ-glutamyl-p-nitroanilide as a substrate?

It is commonly used in γ-glutamyltransferase assay systems and has also appeared in related glutamyl-enzyme research.

Why must the hydrate state be checked?

Commercial catalogs may list anhydrous and hydrated forms differently. Formula, molecular weight, and handling information should match the lot-specific supplied material.

Research Use Only.