$154.00 - $769.00
Fmoc-D-Ser(tBu)-OH is an Fmoc-protected D-serine building block with the side-chain hydroxyl protected as a tert-butyl ether. The tBu group protects the alcohol during peptide assembly while remaining stable to standard Fmoc deprotection conditions.
The side-chain protecting group is removed during acidic final cleavage, restoring the D-serine hydroxyl. This derivative is useful for D-serine incorporation, stereochemical peptide scans and sequences in which hydroxyl orientation influences binding, hydrogen bonding or downstream modification. Its protected form should not be confused with unprotected Fmoc-D-Ser-OH.
Product Information
| Product Name | Fmoc-D-Ser(tBu)-OH |
| Catalog No. | AS3036 |
| CAS No. | 128107-47-1 |
| Molecular Formula | C22H25NO5 |
| Molecular Weight | 383.44 g/mol |
| Protecting Groups | Fmoc alpha-amino protection; O-tBu hydroxyl protection |
| Primary Application | D-serine incorporation in Fmoc-SPPS |
Application Scope and Assay Relevance
Primary use: Protected D-serine building block for stereochemical scanning and cyclic/bicyclic peptide optimization; O-tert-butyl protection masks the hydroxyl during SPPS.
| Typical in vitro relevance | A documented application of D-Ser substitution is cyclic/bicyclic peptide optimization, where D-Ser can improve target inhibition and proteolytic stability when its preferred backbone geometry matches the bound conformation. Enzyme-inhibition, binding, protease-stability and structural assays are therefore relevant downstream uses. |
| In vivo relevance | Animal studies apply to the final optimized peptide. Improvements observed in vitro should be confirmed for exposure, efficacy and tolerability before being attributed to D-Ser substitution. |
tBu protects the serine hydroxyl during chain assembly
O-tert-butyl protection prevents the serine hydroxyl from participating in unwanted side reactions during standard Fmoc-SPPS and is designed for acid-labile removal at final cleavage.
Purchasing and QC Considerations
Confirm CAS 128107-47-1 and the O-tBu protecting group. Reference peptide-synthesis grades report separate chiral-purity and HPLC specifications.
Frequently Asked Questions
What does tBu protect?
It protects the D-serine side-chain hydroxyl.
When is tBu removed?
The ether is normally cleaved during acidic global deprotection.
Is this the same as Fmoc-D-Ser-OH?
No. Fmoc-D-Ser(tBu)-OH contains an additional side-chain tert-butyl protecting group.
Product Documents
A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.
Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.
Related Technical Resources
See Fmoc-D-Thr(tBu)-OH for a closely related building block or synthesis resource.
Browse the complete D-Form Amino Acids category or use Alan Scientific's Chemical Peptide Synthesis service for custom peptides containing D-amino acids.