Boc-His(Trt)-Aib-OH is a protected dipeptide building block containing L-histidine and Aib (alpha-aminoisobutyric acid). The histidine alpha amino group is Boc-protected, the imidazole side chain is Trt-protected, and the C-terminal Aib carboxyl group remains available for further coupling.

The His-Aib motif is especially relevant to semaglutide-related synthesis and impurity research, because semaglutide begins with a His-Aib N-terminal sequence. This product is a protected peptide intermediate, not semaglutide API.

Product Information

Why the His-Aib Motif Is Important

Aib is an alpha,alpha-disubstituted noncanonical amino acid with no chiral center. Incorporation of Aib can strongly influence local conformation and proteolytic susceptibility. In GLP-1 analog design, an Aib residue at position 2 is a well-known strategy for reducing DPP-4 susceptibility of the final peptide.

Semaglutide-Related Synthesis

Because the N-terminal sequence of semaglutide contains His-Aib, a protected His-Aib fragment can be useful in route development, fragment coupling, impurity synthesis and preparation of analytical reference materials related to semaglutide manufacture or research.

The product is not semaglutide itself and does not contain the complete peptide sequence, side-chain lipidation motif or final drug architecture.

Protection Strategy

Boc protects the histidine alpha amino group, while Trt protects the histidine imidazole. The C-terminal Aib carboxyl group can be activated for coupling to the next residue or fragment. Researchers should select deprotection and coupling conditions that match the overall route rather than assuming a standard Fmoc-only workflow.

Applications and Assay Relevance

Applications include semaglutide-related intermediate synthesis, peptide impurity synthesis, analytical standard preparation, GLP-1 analog route development and noncanonical peptide fragment assembly. Biological assays apply to the completed peptide or impurity standard as appropriate; Boc-His(Trt)-Aib-OH itself is an upstream protected intermediate.

Product Documents

A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.

MSDS_Boc-His-Trt--Aib-OH_AS4048

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

Is Boc-His(Trt)-Aib-OH a semaglutide intermediate?

It is a semaglutide-related protected His-Aib building block that can be used in synthesis or impurity/reference-material workflows. It is not semaglutide API.

Is Aib chiral?

No. Aib has two methyl substituents at the alpha carbon and therefore does not have an alpha-carbon stereocenter.

Why is Aib important in GLP-1 analogs?

In the context of a complete peptide, Aib at position 2 can reduce susceptibility to DPP-4 cleavage and is a key noncanonical-residue strategy used in long-acting GLP-1 analog design.

Related Technical Resources

Explore Specialty Peptide Building Blocks and our overview of Noncanonical Amino Acids in Peptide Drug Discovery.

Research Use Only.