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Ac-YVAD-AMC is a fluorogenic tetrapeptide substrate widely used in caspase-1 research. It contains the Tyr-Val-Ala-Asp recognition sequence linked to AMC for fluorescence-based measurement of proteolytic activity.
The YVAD motif has a long history in inflammatory caspase research and is commonly used in studies involving caspase-1 activation, inflammasome biology and enzyme inhibition.
Product Information
| Property | Specification |
|---|---|
| Product Name | Ac-YVAD-AMC |
| Catalog No. | AS2654 |
| CAS No. | 149231-65-2 |
| Sequence | Ac-Tyr-Val-Ala-Asp-AMC |
| Sequence Shortening | Ac-YVAD-AMC |
| Peptide Length | 4 amino-acid residues |
| Molecular Formula | C33H39N5O10 |
| Molecular Weight | Approximately 665.70 Da |
| Reporter Group | 7-Amino-4-methylcoumarin (AMC) |
| Primary Research Enzyme | Caspase-1 / ICE |
Inflammatory Caspase Research
Caspase-1 is a cysteine protease involved in processing inflammatory cytokine precursors and in inflammasome-associated signaling. YVAD-based substrates provide a convenient biochemical readout of YVAD-cleaving enzyme activity.
Related inflammatory caspases can display overlapping tetrapeptide preferences. For cell lysates and complex biological samples, we recommend confirming caspase-1 involvement with suitable inhibitors, genetic controls or complementary assays.
Fluorogenic Readout
Cleavage after Asp releases free AMC and increases fluorescence. This enables sensitive kinetic measurement using a fluorescence plate reader or spectrofluorometer.
Protecting fluorogenic substrates from unnecessary light exposure and maintaining consistent assay timing can improve reproducibility between experimental groups.
Research Applications
Ac-YVAD-AMC can support purified caspase-1 assays, inflammasome-related research, inhibitor evaluation and development of fluorescence-based protease methods.
Related chromogenic and fluorogenic reagents are available in our Caspase Substrates & Inhibitors collection.
Frequently Asked Questions
What does ICE mean in caspase-1 research?
ICE refers to interleukin-1β-converting enzyme, an earlier name for caspase-1.
What is measured after Ac-YVAD-AMC cleavage?
The assay measures fluorescence from liberated AMC.
Is YVAD cleavage absolutely specific to caspase-1?
No. Related proteases can show overlapping substrate preferences, particularly in complex biological samples.