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Ac-YEVD-pNA is an acetylated YEVD chromogenic substrate used in caspase-1/ICE research. The peptide is coupled to p-nitroaniline, providing an absorbance signal after cleavage at the C-terminal Asp.
This format combines a YEVD recognition sequence with simple colorimetric detection and can be used when fluorescence-based instrumentation is not required.
Product Information
| Property | Specification |
|---|---|
| Product Name | Ac-YEVD-pNA |
| Catalog No. | AS2652 |
| Sequence | Ac-Tyr-Glu-Val-Asp-pNA |
| Sequence Shortening | Ac-YEVD-pNA |
| Peptide Length | 4 amino-acid residues |
| Molecular Formula | C31H38N6O12 |
| Molecular Weight | Approximately 686.67 Da |
| Reporter Group | p-Nitroaniline (pNA) |
| Research Context | Caspase-1 / ICE activity studies |
Chromogenic YEVD Cleavage
Enzymatic hydrolysis releases pNA from the peptide, generating a yellow chromophore that can be quantified by absorbance, typically near 405 nm.
The colorimetric format is useful for plate-based endpoint assays, enzyme characterization and comparisons where a direct optical-density measurement is preferred.
Substrate Specificity and Interpretation
YEVD is efficiently recognized by caspase-1 in established peptide-substrate profiling experiments, but caspase substrate recognition is not completely exclusive. Other family members can process related sequences to varying degrees.
We recommend interpreting a pNA signal as peptide-cleaving activity first and assigning a specific enzyme only when the experimental design provides additional supporting evidence.
Choosing Between YEVD-pNA and YEVD-AMC
The peptide-recognition sequence is shared, while the reporter chemistry differs. pNA supports absorbance-based detection, whereas AMC provides a fluorescence readout with generally greater analytical sensitivity.
Researchers can review additional formats in our Caspase Substrates & Inhibitors collection.
Frequently Asked Questions
What is released from Ac-YEVD-pNA after cleavage?
The reporter released is p-nitroaniline.
What is the main difference between Ac-YEVD-pNA and Ac-YEVD-AMC?
They use the same YEVD recognition sequence but different reporter groups: pNA for absorbance and AMC for fluorescence.
Can custom chromogenic substrates be synthesized?
Alternative protease-recognition sequences and reporter conjugates can be evaluated through Chemical Peptide Synthesis.