$362.00 - $362.00
Ac-VDVAD-AMC is a fluorogenic pentapeptide substrate commonly used to monitor caspase-2 activity. The VDVAD recognition sequence is coupled to the fluorescent leaving group AMC.
Following cleavage, free AMC can be detected fluorometrically, enabling sensitive measurement of protease activity in biochemical and apoptosis-related studies.
Product Information
| Property | Specification |
|---|---|
| Product Name | Ac-VDVAD-AMC |
| Catalog No. | AS2655 |
| Sequence | Ac-Val-Asp-Val-Ala-Asp-AMC |
| Sequence Shortening | Ac-VDVAD-AMC |
| Peptide Length | 5 amino-acid residues |
| Molecular Formula | C33H44N6O12 |
| Molecular Weight | Approximately 716.8 Da |
| Reporter Group | 7-Amino-4-methylcoumarin (AMC) |
| Primary Research Enzyme | Caspase-2 |
| Research Areas | Caspase-2 activity, apoptosis, enzyme kinetics, protease assays |
VDVAD as a Caspase-2 Research Substrate
VDVAD-based substrates are widely used for investigating caspase-2 activity, including studies with recombinant enzyme and apoptosis-related experimental models.
Other caspases can also recognize related acidic sequences. We recommend avoiding interpretation of VDVAD cleavage as absolute biochemical specificity without supporting controls.
Fluorescence Measurement
Enzymatic cleavage liberates AMC from the C-terminal Asp. Free AMC provides a fluorescence signal commonly detected in the near-UV excitation and blue-emission range.
The fluorogenic format is useful for kinetic measurements because signal can be collected repeatedly during the course of the reaction.
Assay Planning
Substrate concentration can influence apparent enzyme activity and should be kept consistent when samples are compared. Enzyme source, ionic conditions, reducing environment and incubation temperature can also affect cleavage rates.
Our Peptide Quality Control capabilities support analytical characterization according to the selected product specification.
Frequently Asked Questions
What is the fluorophore in Ac-VDVAD-AMC?
The reporter is 7-amino-4-methylcoumarin, commonly abbreviated AMC.
Is VDVAD used for caspase-2?
Yes. VDVAD-based substrates are widely used to investigate caspase-2 activity.
Can related caspase-2 substrate analogs be prepared?
Sequence variants and reporter modifications can be evaluated through Chemical Peptide Synthesis.