Ac-Gly-Gly-NH2; CAS 27440-00-2 is a terminally capped diglycine model with N-terminal acetylation and a C-terminal primary amide. Both terminal ionizable groups are blocked relative to free Gly-Gly.

This makes the molecule useful as a compact peptide-bond and hydrogen-bonding model, as well as a defined analytical reference for studies that aim to reduce terminal-charge effects.

Product Information

Terminal Capping and Peptide-Bond Modeling

Acetylation and amidation reduce the terminal charge states present in free Gly-Gly. The resulting molecule is a convenient minimal model for peptide-bond solvation, hydrogen bonding and chromatographic behavior.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Fit and Sample Preparation

For comparative or quantitative work, review the lot-specific purity method together with identity data. Chromatographic purity is method-dependent and should be interpreted separately from molecular-mass confirmation or gravimetric vial content.

Solvent and working concentration should be validated for the intended assay. Solution history can influence small peptide models through aggregation, oxidation or concentration-dependent behavior even when the dry material meets its release specification.

Product Documents

MSDS - Ac-Gly-Gly-NH2 (AS4138)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is Ac-Gly-Gly-NH2?

It is N-acetylglycylglycinamide; Ac-Gly-Gly-NH2, a defined research compound with sequence or structural identity Ac-Gly-Gly-NH2.

What is the main value of this product?

Its main value is as a chemically defined reference for terminally capped peptide-bond model.

What should be checked before quantitative use?

Confirm lot-specific identity, purity basis and analytical documentation appropriate to the method.

Research Use Only.