Synthetic Peptide Library

Alan Scientific provides high-throughput custom peptide library synthesis for screening, epitope mapping, target validation, and structure–activity studies, including overlapping, positional, alanine-scanning, truncated, T-cell, and scrambled peptide libraries.

Alan Scientific provides high-throughput custom peptide library synthesis for peptide screening, epitope mapping, target validation, structure–activity relationship (SAR) studies, immunology, and drug discovery.

Peptide libraries can be designed using different sequence strategies depending on the research objective, including overlapping, positional, alanine-scanning, truncation, T-cell epitope, and scrambled peptide libraries.

Peptide Library Synthesis Specifications

Synthesis Format	96-well high-throughput peptide synthesis
Peptide Length	2–30 amino acids
Typical Quantity	1–4 mg per peptide
Typical Turnaround	Approximately 2 weeks
Average Accuracy	>99%
Quality Control	HPLC and mass spectrometry options available
Additional Options	Special purity, modifications, TFA removal, endotoxin control, and project-specific testing

Specifications may vary depending on peptide sequence, library size, purity, modifications, and project requirements.

Peptide Library Design Options

Overlapping Peptide Library

An overlapping peptide library divides a protein or peptide sequence into a series of partially overlapping fragments. The peptide length and overlap can be adjusted according to the experimental design.

This format is commonly used for epitope mapping, antigen screening, protein-region mapping, and identification of biologically active sequence regions.

Overlapping peptide library design for epitope mapping

Positional Peptide Library

A positional peptide library systematically substitutes amino acids at selected positions within a peptide sequence while the remaining sequence is kept constant.

This approach is useful for sequence optimization, residue-tolerance studies, and structure–activity relationship analysis.

Positional peptide library for amino acid substitution and sequence optimization

Alanine Scanning Peptide Library

An alanine scanning peptide library systematically replaces individual residues with alanine while preserving the rest of the peptide sequence.

By comparing the activity of each variant with the original peptide, researchers can identify amino acid residues that are important for binding, recognition, or biological activity.

Alanine scanning peptide library for identification of key amino acid residues

Truncation Peptide Library

A truncation peptide library contains progressively shortened versions of a parent peptide generated by removing residues from the N-terminus, C-terminus, or both termini.

Truncation analysis can help identify the minimum sequence required for binding or biological activity and can be used together with alanine scanning during peptide optimization.

Truncation peptide library for minimum active sequence identification

T-Cell Epitope Peptide Library

A T-cell epitope peptide library uses a series of overlapping peptide sequences derived from a target protein to evaluate potential T-cell recognition regions.

This strategy is commonly used in T-cell epitope mapping, immunology, vaccine research, and immune-response studies.

T-cell epitope peptide library using overlapping peptide sequences

Scrambled Peptide Library

A scrambled peptide library contains sequence variants in which the amino acids of the original peptide are rearranged while maintaining the same or similar overall amino acid composition.

Scrambled peptides are useful for evaluating sequence specificity, experimental controls, and the relationship between amino acid order and peptide activity.

Scrambled peptide library for sequence specificity and experimental controls

Choosing the Right Peptide Library

Research Goal	Recommended Library Strategy
Epitope or active-region mapping	Overlapping Peptide Library
Identify key amino acid residues	Alanine Scanning Library
Optimize individual sequence positions	Positional Peptide Library
Determine the minimum active sequence	Truncation Peptide Library
Map potential T-cell epitopes	T-Cell Epitope Peptide Library
Create sequence-specific controls	Scrambled Peptide Library

Custom Library Options

Peptide libraries can be customized according to sequence, peptide length, number of peptides, quantity, purity, terminal modifications, fluorescent labeling, biotinylation, D- or non-natural amino acids, and other project-specific requirements.

TFA removal, endotoxin control, and additional analytical requirements can also be discussed when needed.

For peptide analytical information, visit ourPeptide Quality Control Report.

Start Your Peptide Library Project

Send us your target protein or peptide sequence, preferred library strategy, peptide length, overlap or substitution requirements, quantity, purity, and modifications for project evaluation.

If the optimal library design has not yet been determined, our scientific team can review the research objective and help select an appropriate peptide library strategy.

Explore ourCustom Peptide Synthesiscapabilities for individual peptide projects.