Chemical Peptide Synthesis
Alan Scientific provides custom peptide synthesis services for linear, cyclic, modified, and long peptides up to 140 amino acids. Flexible purity levels, peptide purification, TFA removal, endotoxin control, HPLC/MS quality control, and a wide range of peptide modifications are available for research and drug discovery applications.
Alan Scientific provides custom peptide synthesis services for research and drug discovery, supporting linear, cyclic, modified, and complex peptides across a broad range of sequence lengths, quantities, and purity requirements.
Our peptide synthesis capabilities include peptides up to 140 amino acids, synthesis scales from µg to kg, purity specifications from desalted material to ≥99%, and a wide range of peptide modifications. Project-specific options such as TFA removal, acetate or hydrochloride salt exchange, endotoxin control, peptide solubility testing, conjugation, D-amino acids, and non-natural amino acids are also available.
Each peptide project can be evaluated according to sequence, quantity, purity, modification, and downstream application requirements.
Custom Peptide Synthesis Specifications
| Specification | Available Options |
|---|---|
| Peptide Types | Linear, cyclic, modified, and specialty peptides |
| Peptide Length | Up to 140 amino acids |
| Synthesis Scale | µg to kg |
| Purity Options | Desalted, ≥75%, ≥80%, ≥85%, ≥90%, ≥95%, ≥98%, ≥99% |
| D-Amino Acids | Partial and all-D peptide synthesis available |
| Non-Natural Amino Acids | Available |
| Peptide Cyclization | Disulfide and head-to-tail cyclization |
| Fluorescent Labeling | Available |
| Biotinylation | Available |
| Protein Conjugation | KLH and BSA conjugation |
| Stable Isotope Labeling | Available |
| Solubility Testing | Available upon request |
| TFA Removal & Counterion Exchange | Reduced TFA (<1% when specified); acetate and hydrochloride (HCl) salt forms available upon request |
| Endotoxin Control | Available; <0.01 EU/µg when specified |
| Quality Control | Analytical HPLC, mass spectrometry, and COA |
Peptide Synthesis Capabilities
Alan Scientific supports both routine peptide synthesis and projects involving difficult sequences, specialized building blocks, cyclization, labeling, or conjugation.
Linear Peptides
Custom linear peptides can be synthesized across a broad range of sequence lengths, quantities, and purity specifications for biochemical, cellular, pharmaceutical, and life science research.
Cyclic Peptides
Cyclic peptide synthesis is available using strategies including disulfide-bond formation and head-to-tail cyclization. The appropriate cyclization method depends on peptide sequence, reactive-group positioning, and the desired molecular structure.
Long and Complex Peptides
Peptides up to 140 amino acids can be evaluated for custom synthesis.
Long, hydrophobic, aggregation-prone, or otherwise difficult peptide sequences may require additional synthesis and purification optimization. Sequence feasibility can be reviewed before production.
D-Amino Acid Peptides
Alan Scientific supports incorporation of individual D-amino acids as well as synthesis of peptides composed entirely of D-amino acids.
Modified Peptides
A broad range of chemical modifications can be incorporated during or after peptide synthesis, depending on sequence design, modification site, and project requirements.
Peptide Conjugates
Peptide conjugation to carrier proteins such as KLH and BSA is available for antibody production, immunological studies, and other research applications.
Peptide Modification Options
Chemical modification allows synthetic peptides to be adapted for specific biological, analytical, imaging, binding, and drug-discovery applications.
Alan Scientific supports a broad range of peptide modifications and specialty amino acid building blocks.
| Modification Category | Common Options | Typical Applications |
|---|---|---|
| N-Terminal Modifications | Acetylation, formylation, fatty-acid modification and other terminal groups | Stability, charge adjustment, structural studies |
| C-Terminal Modifications | Amidation and other C-terminal modifications | Charge adjustment, peptide stability, native peptide mimicry |
| Fluorescent Labeling | FITC, 5-FAM, Dansyl and other fluorescent dyes | Imaging, cellular uptake, binding assays, localization studies |
| Fluorescent Reporters | MCA, AMC, pNA and related reporter groups | Enzyme assays, protease studies, substrate analysis |
| Fluorophore–Quencher Pairs | Quenched fluorescent peptide designs | Protease assays, enzyme activity studies |
| Biotinylation | N-terminal biotin, side-chain biotin and spacer-assisted biotinylation | Pull-down assays, affinity studies, detection and immobilization |
| Phosphorylation | Phosphoserine, phosphothreonine, phosphotyrosine | Cell signaling, kinase studies, protein interaction research |
| D-Amino Acids | D-Ala, D-Arg, D-Asp, D-Cys, D-Leu and other D-amino acids | Stereochemical studies, stability and peptide optimization |
| All-D Peptides | Peptides composed entirely of D-amino acids | Peptide stability, protease-resistance and research applications |
| Non-Natural Amino Acids | Nle, Nva, Aib, Abu, Ornithine and other specialty residues | Structure–activity studies, peptide optimization |
| N-Methyl Amino Acids | N-methylated amino acid building blocks | Conformational control and peptide optimization |
| Stable Isotope Labeling | Stable isotope-labeled amino acids | Quantitative analysis, MS studies, metabolic and structural research |
| Lipid & Fatty-Acid Modification | Fatty-acid and lipid-related conjugation | Membrane association, peptide delivery and pharmacology research |
| Chelator / Functional Groups | DTPA, HYNIC and related functional groups | Imaging, labeling and specialized research applications |
| Disulfide Cyclization | Intramolecular disulfide-bond formation | Structural stabilization and cyclic peptide studies |
| Head-to-Tail Cyclization | N-to-C terminal cyclization | Conformational restriction and cyclic peptide development |
| KLH Conjugation | Peptide–KLH conjugates | Antibody production and immunization studies |
| BSA Conjugation | Peptide–BSA conjugates | Antibody characterization, assay development and research |
| Multiple Antigenic Peptides (MAPs) | Branched multiple-antigen peptide systems | Immunological studies and antigen presentation |
| Custom Modifications | Project-specific chemical groups and specialty building blocks | Application-dependent peptide research |
Modification feasibility can depend on peptide sequence, modification position, peptide length, purification requirements, and compatibility between functional groups. For specialized projects, modification strategy can be evaluated together with the peptide sequence before synthesis.
Peptide Purification and Purity Options
Alan Scientific provides peptide purity specifications ranging from desalted material to ≥99% purity.
Purification requirements depend on the peptide sequence, synthesis complexity, modification, and intended downstream application. More challenging peptides may require additional purification method development to achieve the requested purity and recovery.
For application-specific guidance, see our Recommended Peptide Purity page.
For detailed technical information about RP-HPLC and peptide purification strategy, visit the Peptide Purification & Quality Control Knowledge Center.
Peptide Quality Control
Quality control documentation is provided to support evaluation of peptide purity and molecular identity.
Analytical HPLC is used to evaluate chromatographic purity, while mass spectrometry is used to confirm that the observed molecular mass is consistent with the expected peptide.
A Certificate of Analysis (COA) provides relevant product and quality-control information associated with the delivered peptide.
Representative analytical data are available on our Peptide Quality Control Report page.
Specialized Peptide Services
Alan Scientific supports additional peptide services for projects requiring specialized synthesis, purification, or downstream specifications.
Peptide Library Synthesis
Custom peptide library synthesis is available for peptide screening, epitope mapping, alanine scanning, structure–activity studies, target validation, and immunological research.
Available library strategies include overlapping, positional, alanine-scanning, truncated, T-cell, and scrambled peptide libraries.
TFA Removal & Counterion Exchange
Alan Scientific provides TFA removal and peptide counterion exchange for applications where residual trifluoroacetate may interfere with downstream experiments.
Peptides can be supplied with reduced TFA levels (<1% when specified) or converted to alternative salt forms, including:
Acetate salt
Hydrochloride (HCl) salt
The appropriate peptide salt form can be selected according to the downstream application and project requirements. Please specify any TFA-removal or counterion-exchange requirements when requesting a quotation.
Endotoxin Control
Controlled-endotoxin peptide preparation is available for projects with specific biological requirements. Endotoxin specifications below 0.01 EU/µg can be requested.
Peptide Solubility Testing
Peptide solubility testing is available upon request, particularly for hydrophobic or modified peptides and projects requiring a defined solvent or peptide concentration.
Additional practical information about peptide handling and reconstitution is available in our Peptide Protocols & Technical Guides.
Peptides in Published Research
Alan Scientific peptides have been used in published research across areas including chemical biology, cancer research, immunology, molecular biology, and biomedical research.
These publications provide examples of synthetic peptides used in peptide–protein interaction studies, cellular assays, immunological research, molecular recognition, mechanistic studies, and other experimental applications.
Explore our Publications & Research References for published research involving peptides supplied or synthesized by Alan Scientific.
Technical Resources for Peptide Research
Our Knowledge Center provides technical information covering peptide synthesis, purification, modifications, building blocks, handling, and experimental applications.
Peptide Purification & Quality Control
Peptide Modifications & Applications
Amino Acids & Peptide Building Blocks
Peptide Protocols & Technical Guides
For common questions about peptide synthesis, purity, conjugation, D-amino acids, solubility, storage, purification, and SPPS, visit our Peptide Synthesis Q&A.
Start Your Custom Peptide Project
Send us your peptide sequence, required quantity, target purity, modifications, and intended application for project evaluation.
Providing information about the downstream application can help determine whether additional requirements such as peptide purification, TFA removal, endotoxin control, solubility testing, conjugation, or specialized modifications should be considered.