Fmoc-Glu(OtBu)-Glu(OtBu)-Gly-OH is a protected Glu-Glu-Gly tripeptide. Both glutamate side-chain carboxyl groups are masked as tert-butyl esters, while the terminal Gly carboxyl remains free for further coupling.

This fragment allows a charge-dense acidic motif to be introduced in protected form before global acid deprotection regenerates the two Glu side chains.

Product Information

Application Scope in Acidic Peptide Motifs

Preassembled Glu-Glu-Gly can be useful in acidic protein-mimetic sequences, linker regions and repeated-motif libraries. OtBu protection suppresses side-chain carboxyl reactivity while the fragment is being coupled.

Fragment Assembly and Deprotection

The two Glu side chains remain protected through Fmoc cycles and are regenerated during final acid treatment. The terminal Gly provides a less hindered coupling handle than a β-branched or α,α-disubstituted residue.

Procurement and QC

Confirm formula C35H45N3O10, MW 667.80 g/mol, sequence order and both Glu(OtBu) protections using lot-specific analytical data.

For acidic or repeated-motif sequences, fragment versus stepwise assembly can be compared through custom peptide synthesis.

Product Documents

A Safety Data Sheet (SDS / MSDS) is available for this product to support laboratory handling, storage and safety assessment.

Fmoc-Glu(OtBu)-Glu(OtBu)-Gly-OH MSDS

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

How many Glu side chains are protected?

Two, both as OtBu esters.

Which carboxyl is used for further coupling?

The C-terminal Gly carboxyl.

Related Technical Resources

Review the SPPS workflow

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