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Ac-Hyp-OH is N-acetyl-trans-4-hydroxy-L-proline, an N-blocked hydroxyproline derivative with a free carboxyl group and a native 4-hydroxyl substituent on the pyrrolidine ring.
N-acetylation changes the nitrogen from a reactive secondary amine into an amide. The material is therefore better viewed as a defined hydroxyproline derivative, reference compound, or synthetic intermediate than as a conventional N-protected residue for iterative peptide-chain extension.
Product Information
| Product Name | Ac-Hyp-OH |
| Chemical Name | N-Acetyl-trans-4-hydroxy-L-proline |
| Catalog No. | AS2088 |
| CAS No. | 33996-33-7 |
| Molecular Formula | C7H11NO4 |
| Molecular Weight | 173.17 g/mol |
| Compound Type | N-acetylated hydroxyproline derivative |
| Primary Research Use | Hydroxyproline reference/intermediate and solution-phase synthetic chemistry |
Hydroxyproline and N-Acetylation
Hydroxyproline introduces a ring-constrained amino-acid framework with an additional hydroxyl function. Acetylating the ring nitrogen removes its normal nucleophilicity and fixes the N-acyl state.
That identity is useful in reference-material work, derivatization studies, and routes where the N-acetyl group is part of the desired product or intermediate.
Synthetic Use and Selection
The carboxyl group remains available for esterification, salt formation, activation, or other transformations. The hydroxyl group may also participate in derivatization if the reaction is designed for it.
For chain-extendable protected residues, compare other specialty amino-acid building blocks rather than assuming Ac-Hyp-OH behaves like an Fmoc-Hyp derivative.
Procurement Considerations
CAS 33996-33-7, stereochemical naming, purity, and lot analytical data should be checked together. Hydroxyproline products can differ in cis/trans configuration, N-protection state, and esterification state.
Product Documents
The Safety Data Sheet provides product identification, hazard information, handling and storage guidance, exposure controls, transport information, and regulatory information for laboratory use.
Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.
Frequently Asked Questions
Is Ac-Hyp-OH an N-protected hydroxyproline?
It is N-acetylated hydroxyproline. The acetyl group is an amide and is not used like a routine temporary Fmoc protecting group.
Is the carboxyl group free?
Yes. The carboxyl group is not esterified in Ac-Hyp-OH.
Why does cis/trans hydroxyproline matter?
The relative stereochemistry of the hydroxyl and carboxyl-bearing centers changes the three-dimensional structure and distinguishes different hydroxyproline derivatives.