Rink Amide AM Resin is an acid-labile solid support designed for the Fmoc solid-phase peptide synthesis of C-terminally amidated peptides. The modified Rink amide linker is attached to an aminomethyl-polystyrene support, providing a practical route to peptide carboxamides using standard Fmoc deprotection and coupling cycles.

After peptide-chain assembly, strong acidic cleavage releases the peptide with a C-terminal amide rather than a free carboxylic acid. This makes Rink Amide AM Resin especially useful for synthetic analogs of naturally amidated peptide hormones, neuropeptides, receptor ligands and other research peptides in which terminal amidation is part of the required molecular identity.

Product Information

Rink Amide Resin for C-Terminal Peptide Amides

Choice of solid support determines the terminal functionality generated after cleavage. Rink Amide AM Resin is selected when the target sequence requires a primary C-terminal amide, whereas peptide-acid supports such as Wang or 2-Chlorotrityl resin generate a C-terminal carboxylic acid.

This distinction is chemically important because terminal amidation removes the negative charge associated with a free C-terminal carboxylate and can materially affect peptide conformation, receptor recognition, stability and biological activity.

Fmoc-SPPS Workflow

The resin is compatible with standard Fmoc-SPPS cycles. Following removal of the initial Fmoc group from the linker, the first Fmoc-protected amino acid is coupled to the available amine, followed by iterative Fmoc deprotection and amino-acid coupling.

At the end of synthesis, TFA-based cleavage releases the peptide amide and can simultaneously remove conventional acid-labile side-chain protecting groups, depending on the cleavage cocktail used.

Rink Amide AM Resin vs Peptide-Acid Resins

Use Rink Amide AM Resin when the required product is peptide-NH₂. If the required product is peptide-COOH, a resin such as Wang Resin or 2-Chlorotrityl Chloride Resin is generally more appropriate.

For another Rink amide support option, see Rink Amide-MBHA Resin. Compare lot-specific loading and physical specifications when evaluating the two products for your synthesis.

Applications

Rink Amide AM Resin is suitable for synthesis of amidated neuropeptides, peptide hormones, receptor ligands, antimicrobial peptides, peptide libraries, modified peptides and other sequences where a C-terminal carboxamide is required.

The resin is an upstream synthetic material rather than a direct biological assay reagent. The resulting purified peptide amides may subsequently be evaluated in receptor-binding assays, enzyme assays, cell-based studies, antimicrobial assays or in vivo research as appropriate for the final peptide.

Loading, Mesh Size and Procurement

Commercial Rink Amide AM resins are available in different loading ranges and particle sizes. These parameters influence synthesis scale, reagent equivalents, swelling behavior and resin handling.

Alan Scientific recommends using the lot-specific loading value from the Certificate of Analysis rather than assuming a generic mmol/g value from the resin name or CAS number.

For guidance on evaluating loading alongside sequence, support and solvent conditions, see Peptide Resin Loading in SPPS: How to Choose the Right Loading.

Product Documents

A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.

MSDS_Rink-Amide-AM-Resin_AS2061

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What type of C-terminus does Rink Amide AM Resin produce?

It produces a primary C-terminal peptide amide after cleavage.

Is Rink Amide AM Resin compatible with Fmoc-SPPS?

Yes. It is specifically designed for Fmoc solid-phase peptide synthesis.

What is the difference between Rink Amide Resin and Wang Resin?

Rink Amide Resin produces peptide amides, whereas Wang Resin normally produces peptide carboxylic acids.

Does every Rink Amide AM Resin have the same loading?

No. Loading and mesh size vary by grade and lot and should be confirmed from the current COA.

Related Technical Resources

Browse additional Peptide Synthesis Resins.

For complete synthesis of C-terminally amidated peptides, see our Chemical Peptide Synthesis service.

Research Use Only.