HIV-1 Tat (49–57), sequence RKKRRQRRR, is a 9-amino-acid, highly cationic cell-penetrating peptide (CPP) derived from the basic protein transduction domain of HIV-1 Tat.
Tat(49–57) represents a minimal arginine- and lysine-rich region capable of supporting cellular uptake and is widely used as a reference CPP in intracellular delivery, peptide conjugation, membrane interaction, and structure–activity studies.
Product Information
| Property | Specification |
|---|---|
| Product Name | HIV-1 Tat (49–57) |
| Catalog No. | AS2692 |
| Sequence | RKKRRQRRR |
| Length | 9 amino acids |
| Peptide Class | Cell-penetrating peptide |
| Origin | HIV-1 Tat protein |
| Functional Region | Protein transduction domain |
| Research Areas | CPP research, intracellular delivery, cellular uptake, cargo conjugation |
Minimal TAT Protein Transduction Domain
Tat(49–57) contains eight positively charged arginine or lysine residues within a nine-residue sequence. This strong cationic character supports interactions with negatively charged cell-surface components and contributes to cellular internalization.
Tat-derived CPP uptake can involve several mechanisms depending on concentration, cell type, attached cargo, and experimental conditions. For this reason, cellular uptake should not be considered an intrinsic constant of the free peptide.
Tat(49–57) is particularly useful as a compact reference sequence when comparing newly designed arginine-rich CPPs.
Research Applications
Tat(49–57) can be used in studies involving intracellular peptide and protein delivery, fluorescent probe uptake, nanoparticle functionalization, peptide–membrane interactions, CPP mechanism studies, and cargo conjugation.
Researchers requiring fluorescent labeling, biotinylation, terminal modifications, or cargo conjugation can use our Custom Peptide Synthesis capabilities.
Experimental Considerations
Cargo identity, conjugation position, linker chemistry, peptide concentration, incubation time, and cell type can substantially influence Tat-mediated uptake.
Because Tat(49–57) contains multiple Arg and Lys residues, proteolytic stability should also be considered in biological matrices or prolonged cell-based experiments.
Analytical HPLC and mass spectrometry can be used to evaluate purity and molecular identity. See Peptide Quality Control for additional information.
Frequently Asked Questions
What is the sequence of HIV-1 Tat (49–57)?
The sequence is RKKRRQRRR.
How many amino acids are in Tat(49–57)?
Tat(49–57) contains 9 amino acids.
Is Tat(49–57) a cell-penetrating peptide?
Yes. Tat(49–57) is a classical arginine-rich CPP derived from the HIV-1 Tat protein transduction region.
What is Tat(49–57) used for?
It is commonly used in intracellular delivery, cellular uptake, CPP mechanism, peptide conjugation, and comparative delivery studies.
Research Use Only.
HIV-1 Tat (49–57) is supplied for research use only. It is not intended for therapeutic, or human use.