H-Pro-Gly-Gly-OH (L-prolylglycylglycine; CAS 7561-25-3) is a Pro-Gly-Gly tripeptide with a proline residue followed by two glycines. The sequence provides a small Pro/Gly-rich peptide model with low side-chain complexity.

It can be used in conformational, analytical and collagen-motif model studies, while recognizing that the isolated tripeptide does not reproduce the structure or biological function of a full collagen sequence.

Product Information

Pro/Gly-Rich Sequence Model

Proline and glycine are common components of collagen-like sequence motifs. Pro-Gly-Gly can be used as a minimal structural or analytical model, but a three-residue peptide cannot reproduce the triple-helical behavior of native collagen.

Analytical Identity and Method Development

Short peptides are often purchased as reference compounds because exact sequence, terminal state and stereochemistry can influence retention, ionization and fragmentation. When peak assignment matters, use the exact compound identity rather than relying only on nominal molecular mass.

Procurement and QC Considerations

For research use, match the requested purity and documentation to the experiment. Analytical or quantitative studies benefit from lot-specific HPLC and MS data, while metabolomics or sequence-isomer work may require additional identity controls.

For identity and purity documentation, review our peptide QC methods for HPLC and mass-spectrometric characterization principles.

For broader sequence or project work, see analytical peptide references.

Analytical Use and Lot Documentation

For qualitative identification or method development, sequence identity, expected molecular mass and chromatographic purity may be sufficient. Quantitative use can require additional lot-specific information such as an assigned purity or content basis and a preparation protocol appropriate to the analytical method.

HPLC purity and mass-spectrometric identity answer different questions and should not be treated as interchangeable. Solubility and solution stability should also be validated at the concentration and solvent conditions used in the experiment when no product-specific solution data are available.

Product Documents

MSDS - H-Pro-Gly-Gly-OH (AS4114)

The Safety Data Sheet provides product identification, laboratory handling guidance, exposure controls, transport information and safety information for research use.

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

What is H-Pro-Gly-Gly-OH?

It is L-prolylglycylglycine; Pro-Gly-Gly, a defined research compound with sequence or structural identity Pro-Gly-Gly.

What is the main value of this product?

Its main value is as a chemically defined reference for proline/glycine-rich tripeptide reference.

What should be checked before quantitative use?

Confirm lot-specific identity, purity basis and analytical documentation appropriate to the method.

Research Use Only.