Fmoc-Val-Ala-OH is a preassembled, N-terminally Fmoc-protected L-Val-L-Ala dipeptide with a free C-terminal carboxylic acid. It is used in peptide synthesis and is especially relevant as a building block for protease-cleavable ADC and peptide-drug conjugate linker research.

Val-Ala is an established lysosomally cleavable dipeptide motif used in linker design. The protected fragment allows the Val-Ala sequence to be incorporated as a defined unit, while the Fmoc group supports controlled N-terminal extension after base-mediated deprotection.

Product Information

Val-Ala as a Cleavable Linker Motif

Val-Ala belongs to the class of short peptide motifs explored for lysosomal protease-responsive drug-linker systems. In the final conjugate, peptide cleavage can be combined with a self-immolative spacer such as PAB/PABC to translate enzymatic processing into payload release.

The behavior of Val-Ala is not intrinsic to the protected dipeptide alone. Plasma stability, lysosomal cleavage rate, payload release and cell activity depend on the complete linker, conjugation site, spacer, payload and biological system.

Applications in ADC and Bioconjugation Research

Typical downstream evaluation can include lysosomal or protease-cleavage assays, plasma-stability experiments, LC-MS release studies, cell-based cytotoxicity assays and in vivo pharmacology after the complete conjugate has been synthesized and characterized.

Fmoc-Val-Ala-OH vs Other Val-Ala Building Blocks

Val-Ala fragments may be supplied with different N-terminal protecting groups, including Fmoc or Alloc. These versions contain the same peptide sequence but support different orthogonal synthesis strategies and should not be treated as interchangeable without considering the intended deprotection conditions.

Safety and Procurement Considerations

Current supplier safety information for CAS 150114-97-9 reports H302, H315, H319 and H335. Review the product-specific MSDS and use appropriate laboratory controls.

For procurement, confirm CAS, L-Val-L-Ala stereochemistry, Fmoc protection, free C-terminal acid and lot-specific purity.

Product Documents

A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.

MSDS_Fmoc-Val-Ala-OH_AS4071

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

Is Fmoc-Val-Ala-OH used in ADC linker synthesis?

Yes. Val-Ala is used as a protease-cleavable peptide motif in ADC and related conjugate-linker research.

Does Fmoc-Val-Ala-OH itself have ADC activity?

No. It is an upstream linker building block; activity and cleavage are evaluated on the completed conjugate.

Why buy a preassembled Val-Ala dipeptide?

It fixes the sequence and stereochemical junction and removes one peptide-coupling step from the downstream synthesis.

Related Technical Resources

Explore additional Specialty Peptide Building Blocks.

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