Fmoc-Gly-Val-OH is an Fmoc-protected glycyl-L-valine (Gly-Val, GV) dipeptide with a free C-terminal carboxylic acid. It provides a preassembled junction between flexible glycine and beta-branched L-valine for peptide-fragment synthesis, linker development and sequence-defined conjugation chemistry.

The product is chemically distinct from Val-Gly. Because sequence order controls which residue is N-terminal versus C-terminal, researchers should verify Gly-Val orientation rather than relying only on residue composition or molecular weight.

Product Information

Gly-Val as a Defined Dipeptide Fragment

Glycine provides minimal steric bulk, whereas valine is hydrophobic and beta-branched. Combining them in a preassembled fragment can be useful in routes where direct formation of a junction adjacent to a beta-branched residue is less convenient or where the GV motif is already fixed in the target design.

Using a preassembled dipeptide can reduce one coupling operation and provides a verified L-Val stereocenter before the fragment is incorporated into a longer sequence.

Sequence Orientation Matters: Gly-Val vs Val-Gly

Gly-Val and Val-Gly contain the same two residue types but are not the same molecule. They differ in peptide-bond orientation, terminal residue identity and sequence context. This distinction matters for enzyme recognition, peptide conformation and any downstream biological interpretation.

Procurement searches should therefore use exact terms such as Fmoc-Gly-Val-OH, Fmoc-GV-OH, N-Fmoc-glycyl-L-valine and CAS 86895-14-9.

Application Scope

Fmoc-Gly-Val-OH can be used in SPPS, protected-fragment coupling, linker synthesis, ligation-handle development and preparation of modified or peptidomimetic sequences containing the GV motif.

Final peptides or conjugates may be evaluated in enzyme, binding, cell-based or in vivo studies. The protected dipeptide itself is an upstream synthesis reagent rather than a direct assay compound.

Environmental Handling Note

Public regulatory and supplier records commonly classify Fmoc-Gly-Val-OH as very toxic to aquatic life (H400). Avoid unnecessary environmental release and follow the product-specific MSDS and local waste-disposal requirements.

Purchasing and QC Considerations

Confirm CAS identity, sequence order, L-Val stereochemistry, Fmoc protection and lot-specific purity. Formula and molecular weight alone cannot distinguish all sequence or stereochemical alternatives.

Product Documents

A Material Safety Data Sheet (MSDS) is available for this product to support laboratory handling, storage and safety assessment.

MSDS_Fmoc-Gly-Val-OH_AS4066

Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.

Frequently Asked Questions

Is Fmoc-Gly-Val-OH the same as Fmoc-Val-Gly-OH?

No. The sequence order is reversed, producing a different dipeptide structure.

Why use a preassembled Gly-Val fragment?

It can simplify assembly of a defined GV junction and provides controlled L-Val stereochemistry before the fragment is incorporated into a longer peptide.

Does Fmoc-Gly-Val-OH require special waste handling?

Public hazard records classify it as hazardous to the aquatic environment, so environmental release should be avoided and disposal should follow the MSDS and applicable regulations.

Related Technical Resources

Explore additional Specialty Peptide Building Blocks for protected fragments, linker motifs and peptide-synthesis intermediates.

For sequence-specific synthesis, protected-fragment assembly and modified peptide projects, see our Chemical Peptide Synthesis service.

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