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| Product Name | Fmoc-Ala-OH |
| Synonyms | Fmoc-L-alanine; N-(9-Fluorenylmethoxycarbonyl)-L-alanine |
| CAS Number | 35661-39-3 |
| Molecular Formula | C18H17NO4 |
| Molecular Weight | 311.33 g/mol |
| SMILES | C[C@H](NC(=O)OCC1c2ccccc2-c3ccccc13)C(O)=O |
| Appearance | White to almost white powder or crystalline solid |
| Storage Temperature | 2–8°C |
| Melting Point | 147–153°C (lit.) |
| Functional Groups | Fmoc-protected amine; carboxylic acid |
| Primary Application | Fmoc solid-phase peptide synthesis (SPPS) |
Product Overview
Fmoc-Ala-OH, also known as Fmoc-L-alanine, is an Fmoc-protected L-alanine building block commonly used in Fmoc-based solid-phase peptide synthesis (SPPS). The Fmoc group provides temporary protection of the α-amino group during stepwise peptide chain assembly while leaving the carboxylic acid available for activation and coupling.
Alanine contains a small, nonpolar methyl side chain and does not require an additional side-chain protecting group during standard Fmoc-SPPS. This makes Fmoc-Ala-OH a frequently used building block for routine peptide synthesis, peptide libraries, peptide analog preparation, and sequence optimization.
Applications in Peptide Synthesis
| Application | Role of Fmoc-Ala-OH |
| Solid-Phase Peptide Synthesis | Incorporation of L-alanine during Fmoc-based peptide chain assembly |
| Custom Peptide Synthesis | Preparation of linear, cyclic, modified, and research peptides containing L-alanine |
| Peptide Library Synthesis | Building block for parallel peptide synthesis and library construction |
| Alanine Substitution Studies | Preparation of alanine-substituted peptide analogs for functional and binding studies |
| Sequence Optimization | Generation of peptide analogs for sequence comparison and structure–activity relationship studies |
Fmoc-Ala-OH in Fmoc-SPPS
During Fmoc-SPPS, protected amino acids are sequentially coupled to a resin-bound peptide chain. Fmoc-Ala-OH introduces an L-alanine residue while the α-amino group remains temporarily protected by Fmoc.
Following successful coupling, the Fmoc protecting group can be removed under standard base-mediated deprotection conditions to expose the amino group for the next elongation cycle. This orthogonal protection strategy enables controlled, stepwise peptide assembly.
Because alanine has no reactive side-chain functional group requiring additional protection, Fmoc-Ala-OH is generally considered a straightforward building block in routine peptide synthesis.
Applications in Peptide Design
Alanine is also frequently used when evaluating the contribution of individual amino acid side chains to peptide activity. In an alanine substitution or alanine-scanning strategy, selected residues are replaced by alanine and the resulting peptide analogs are compared experimentally.
This approach can help researchers investigate residues involved in protein–peptide interactions, receptor recognition, enzymatic activity, or other sequence-dependent biological functions.
Product Documents
A Safety Data Sheet (SDS / MSDS) is available to support laboratory handling, storage, and safety assessment of this product.
Related Technical Resources
For additional information on the use of Fmoc-protected amino acids in peptide synthesis, see Solid-Phase Peptide Synthesis (SPPS): A Practical Guide.
Explore additional Standard Fmoc-Amino Acids for peptide synthesis applications.
For projects requiring complete peptide production, Alan Scientific provides Custom Peptide Synthesis including synthesis, purification, modification, and analytical quality control.
Why Source Peptide Building Blocks from Alan Scientific?
We supply peptide building blocks and protected amino acids for research and peptide synthesis applications, with competitive pricing, flexible ordering options, and peptide synthesis technical support.
Researchers can source individual Fmoc amino acids or combine building-block procurement with custom peptide synthesis services according to project needs.