Product Overview

Fmoc-Ala-OH (CAS 35661-39-3; catalog AS2483) is N-Fmoc-L-alanine, a protected amino acid building block for Fmoc solid-phase peptide synthesis and peptide-fragment preparation. The Fmoc group protects the alpha-amino group, while the alpha-carboxyl group remains available for coupling. Alanine has a methyl side chain and requires no side-chain protecting group for conventional Fmoc-SPPS.

Quality Specifications and Ordering

Purity: Alan Scientific supplies Fmoc-Ala-OH at >99% purity.

Catalog pack sizes: 25 g, 100 g, 500 g, and 1 kg.

Quality documentation: See Product Documents below for lot-specific COA request details. Review the analytical methods and results reported for the supplied batch.

Quotation: Email [email protected] with AS2483, the pack size, total quantity, delivery destination, and required date. Confirm availability and dispatch lead time before ordering.

For alanine-scanning panels, estimate total Ala consumption across the planned sequences and synthesis scales. Specify any stereochemical test requirement separately from the >99% purity specification.

Alanine Scanning and Sequence Comparisons

L-Alanine is commonly introduced into substitution panels to investigate the contribution of individual side chains. Replacing a larger residue with Ala removes side-chain functionality while retaining an L-configured alpha-carbon. Such panels can support studies of recognition motifs and peptide structure-activity relationships.

An alanine substitution is not necessarily a neutral change to the backbone. Replacing glycine adds a stereocenter and a methyl group; replacing proline removes its ring constraint. Assay controls and sequence-specific interpretation are therefore important when connecting an observed response to a particular side-chain interaction.

For an alanine-substitution panel rather than the building block alone, review our custom peptide synthesis service and provide the parent sequence and intended variants.

Use in Fmoc Peptide Assembly

Fmoc-Ala-OH is suitable for incorporating L-Ala into defined research peptide sequences and synthetic intermediates. Standard Fmoc removal exposes the amino group for the next coupling step. The lack of a reactive side-chain functionality simplifies protection planning but does not eliminate sequence-dependent incomplete coupling or aggregation.

For parallel synthesis, define the parent sequence, substitution positions, terminal groups, and any labels before preparing the panel. Keeping these features consistent helps separate the effect of the Ala substitution from changes elsewhere in the construct.

Product Documents

A Safety Data Sheet (SDS / MSDS) is available to support laboratory handling, storage, and safety assessment of this product.

MSDS_Fmoc-Ala-OH_AS2483

Frequently Asked Questions

How should I choose a pack size for an alanine-scanning panel?

Estimate the total Ala requirement from all planned sequences, synthesis scales, and coupling equivalents, including an allowance for repeat reactions. Fmoc-Ala-OH is listed in 25 g, 100 g, 500 g, and 1 kg packs. The number of variants alone is not enough to determine reagent consumption.

Is Fmoc-Ala-OH the same as Fmoc-D-Ala-OH?

No. The L- and D-alanine derivatives have different stereochemistry and must be selected according to the intended peptide sequence.

Does an alanine scan prove which residues bind a target?

It provides experimental comparisons, not automatic proof of a binding contact. A substitution can also affect folding, solubility, or other sequence-dependent properties.

Research Use Only

For research use only. Not for human or veterinary use.