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Biotin-Kemptide is a directly biotinylated form of the PKA substrate Kemptide with the sequence Biotin-Leu-Arg-Arg-Ala-Ser-Leu-Gly.
The construct combines the established LRRASLG phosphorylation sequence with an N-terminal biotin group, enabling streptavidin-based capture and detection in kinase assay workflows.
Product Information
| Property | Specification |
|---|---|
| Product Name | Biotin-Kemptide |
| Catalog No. | AS2629 |
| Sequence | Biotin-Leu-Arg-Arg-Ala-Ser-Leu-Gly |
| Core Sequence | LRRASLG |
| Peptide Length | 7 amino acids |
| Molecular Weight | Approximately 998.22 Da |
| Molecular Formula | C42H75N15O11S |
| Modification | N-terminal biotin |
| Phosphorylation Site | Ser5 of the Kemptide sequence |
| Primary Research Enzyme | Protein kinase A (PKA) |
Direct Biotinylation of the Kemptide Substrate
The LRRASLG region retains the serine-containing recognition sequence used in PKA phosphorylation assays, while N-terminal biotin provides a high-affinity handle for streptavidin-based analytical formats.
This configuration can support immobilization, capture and separation of substrate or phosphorylated product without introducing an additional long-chain spacer.
Direct biotinylation changes the N-terminal chemical environment of Kemptide. We therefore recommend confirming assay performance when kinetic parameters from unmodified Kemptide are used as a reference.
Research Applications
Biotin-Kemptide can be incorporated into streptavidin plate assays, phosphorylation detection workflows, kinase activity studies and assay-development platforms where substrate capture is required.
For experiments requiring greater separation between the affinity tag and substrate sequence, Biotin-LC-Kemptide provides an alternative spacer-containing format.
Assay Design Considerations
The biotin–streptavidin interaction simplifies capture but does not itself identify phosphorylation. A compatible phosphopeptide-detection method or other assay readout is still required to distinguish phosphorylated from unmodified substrate.
For quantitative kinase studies, we recommend controlling substrate concentration, enzyme amount, reaction time and ATP conditions rather than assuming that a biotinylated derivative will reproduce the exact kinetics of unmodified Kemptide.
Frequently Asked Questions
Is Biotin-Kemptide a PKA or PKC substrate?
The Kemptide sequence LRRASLG is a well-established substrate for cAMP-dependent protein kinase A (PKA).
What is the purpose of the biotin group?
Biotin allows high-affinity capture or immobilization using streptavidin-based reagents and surfaces.
Can other labels be added to Kemptide?
Alternative affinity tags, fluorescent labels and sequence modifications can be evaluated through Chemical Peptide Synthesis.