Ac-DEVD-pNA is a colorimetric DEVD peptide substrate used to monitor executioner caspase activity through release of p-nitroaniline.

The DEVD sequence is a well-established recognition motif for caspase-3 and caspase-7, while the pNA reporter provides a convenient absorbance-based alternative to fluorogenic AMC substrates.

Product Information

Colorimetric Detection of DEVD Cleavage

Caspase-mediated hydrolysis releases free pNA from the C-terminal Asp. The liberated chromophore can be monitored near 405 nm, enabling enzyme activity to be quantified using a conventional absorbance reader.

This makes Ac-DEVD-pNA useful for endpoint assays, kinetic measurements and screening workflows where fluorescence detection is unnecessary or unavailable.

Executioner Caspase Research

DEVD-based substrates are strongly associated with caspase-3/-7 activity and are widely used in apoptosis research. They can also be processed by additional cysteine proteases under some conditions.

We therefore recommend reporting the reagent as a DEVD substrate and using biological controls when assigning cleavage to a single caspase.

Choosing pNA or AMC

pNA provides a direct colorimetric readout and simplifies instrumentation. AMC generally offers greater fluorescence sensitivity. The preferred reporter depends on assay scale, sample background and the available detection platform.

Additional formats are available in our Caspase Substrates & Inhibitors collection.

Frequently Asked Questions

At what wavelength is pNA commonly measured?

Released p-nitroaniline is commonly monitored by absorbance near 405 nm.

Is Ac-DEVD-pNA specific only for caspase-3?

No. Caspase-7 and some related proteases can also cleave DEVD-based substrates.

Can reporter groups be changed for a custom assay?

Reporter and sequence modifications can be evaluated through Chemical Peptide Synthesis.

Research Use Only.