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Boc-Gly-Gly-Gly-OH is an N-Boc-protected triglycine fragment with a free C-terminal carboxyl group. It provides a defined three-glycine sequence as a single protected intermediate.
For a shorter Fmoc-protected glycine fragment, compare Fmoc-Gly-Gly-OH. The two products differ in both chain length and N-terminal protection, so they should not be treated as interchangeable linkers.
Product Information
| Product Name | Boc-Gly-Gly-Gly-OH |
| Catalog No. | AS4194 |
| CAS No. | 28320-73-2 |
| Molecular Formula | C11H19N3O6 |
| Molecular Weight | 289.29 g/mol |
| Building Block Type | Boc-protected Gly-Gly-Gly tripeptide fragment |
| Primary Applications | Flexible Gly3 linker synthesis; solution-phase peptide chemistry; fragment coupling; peptide and conjugate intermediates |
Why a Gly3 Fragment Is Useful
Three consecutive glycine residues create a compact, highly flexible peptide spacer with minimal side-chain steric bulk. Gly3 is commonly selected when the goal is separation between functional elements without adding a bulky hydrophobic linker.
Chain Length Is a Design Variable
Gly2, Gly3, Gly4, PEG, and aminohexanoic acid spacers all create different distances, flexibility, polarity, and proteolytic profiles. We recommend choosing the linker for the final construct rather than treating all 'flexible spacers' as equivalent.
Boc Protection Favors Acid-Labile N-Terminal Control
The N-terminal Boc group protects the fragment during carboxyl activation and is removed under acidic conditions. This makes the fragment suitable for solution-phase or Boc-compatible route designs.
Procurement and QC
Confirm CAS 28320-73-2, formula C11H19N3O6, MW 289.29 g/mol, exact Gly-Gly-Gly chain length, and N-terminal Boc protection. LC-MS is useful for excluding nearby oligoglycine homologs.
Whether to introduce Gly3 as a preformed fragment or stepwise sequence can be evaluated within the SPPS workflow and the complete target.
Product Documents
A Safety Data Sheet (SDS / MSDS) is available for this product to support laboratory handling, storage and safety assessment.
Certificates of Analysis (COAs) are batch-specific. Please contact us to request the COA for your product, and we will provide it by email.
Frequently Asked Questions
What does Gly3 mean?
It is a sequence of three consecutive glycine residues.
Is the C-terminal carboxyl free?
Yes. The terminal Gly carboxyl remains available for further coupling.
Why use a preformed triglycine fragment?
It can reduce downstream coupling steps and provides a separately characterized Gly3 unit.
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